Plant Transcription Factor Database
Previous version: v3.0
Arachis duranensis
HSF Family
Species TF ID Description
Aradu.17BB2HSF family protein
Aradu.2K11IHSF family protein
Aradu.43K0PHSF family protein
Aradu.5S8J3HSF family protein
Aradu.95F2QHSF family protein
Aradu.G1NHGHSF family protein
Aradu.H5REBHSF family protein
Aradu.H8ZFNHSF family protein
Aradu.K2DQ9HSF family protein
Aradu.L9WUKHSF family protein
Aradu.MA5WHHSF family protein
Aradu.N1DAKHSF family protein
Aradu.N3N49HSF family protein
Aradu.NTQ7WHSF family protein
Aradu.R0U9BHSF family protein
Aradu.R99HZHSF family protein
Aradu.RFN6QHSF family protein
Aradu.RVF1VHSF family protein
Aradu.RY508HSF family protein
Aradu.S2L37HSF family protein
Aradu.S4DGVHSF family protein
Aradu.U9IPRHSF family protein
Aradu.X3DNXHSF family protein
Aradu.YW6UWHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645